Rolo T.D.S., Ershov A., Van De Kamp T., Baumbach T.
in Proceedings of the National Academy of Sciences of the United States of America, 111 (2014) 3921-3926. DOI:10.1073/pnas.1308650111
Abstract
Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full fourdimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and subsecond time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut-type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.
Onelli O.et al.: Development of structural colour in leaf beetles in Scientific Reports, 7 (2017) 1373.
Handschuh S.et al.: Microscopic dual-energy CT (microDECT): a flexible tool for multichannel ex vivo 3D imaging of biological specimens in Journal of Microscopy, 267 (2017) 3-26.
Zhou G.et al.: A low dose and in-vivo imaging system based on equally sloped tomography in Proceedings – International Symposium on Biomedical Imaging (2017) 60-63 7950468.
Wulff N.et al.: Copulatory courtship by internal genitalia in bushcrickets in Scientific Reports, 7 (2017) 42345.
Hoshino M.et al.: 4D x-ray phase contrast tomography for repeatable motion of biological samples in Review of Scientific Instruments, 87 (2016) 093705.
Sun F.et al.: Investigation of failure mechanisms in silicon based half cells during the first cycle by micro X-ray tomography and radiography in Journal of Power Sources, 321 (2016) 174-184.
Voropaev A.et al.: Direct Fourier Inversion Reconstruction Algorithm for Computed Laminography in IEEE Transactions on Image Processing, 25 (2016) 2368-2378 7440886.
Sun F.et al.: Three-Dimensional Visualization of Gas Evolution and Channel Formation inside a Lithium-Ion Battery in ACS Applied Materials and Interfaces, 8 (2016) 7156-7164.
Yao S.et al.: Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability in Applied Physics Letters, 108 (2016) 123702.
Yang Y.et al.: Contrast transfer functions for Zernike phase contrast in full-field transmission hard X-ray microscopy in Optics Express, 24 (2016) 6063-6070.
Schwermann A.et al.: Preservation of three-dimensional anatomy in phosphatized fossil arthropods enriches evolutionary inference in eLife, 5 (2016) e12129.
Vogelgesang M.et al.: Real-time image-content-based beamline control for smart 4D X-ray imaging in Journal of Synchrotron Radiation, 23 (2016) 1254-1263.
Ibrahimkutty S.et al.: A hierarchical view on material formation during pulsed-laser synthesis of nanoparticles in liquid in Scientific Reports, 5 (2015) 16313.
Zhang M.et al.: Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation in Scientific Reports, 5 (2015) 14982.
Brehm G.et al.: The unique sound production of the Death’s-head hawkmoth (Acherontia atropos (Linnaeus, 1758)) revisited in Science of Nature, 102 (2015) 43.
Yang X.et al.: TV-based conjugate gradient method and discrete L-curve for few-view CT reconstruction of X-ray in vivo data in Optics Express, 23 (2015) 5368-5387.
Mokso R.et al.: Four-dimensional in vivo X-ray microscopy with projection-guided gating in Scientific Reports, 5 (2015) 8727.
Koch M.et al.: Tentorial mobility in centipedes (Chilopoda) revisited: 3D reconstruction of the mandibulo-tentorial musculature of Geophilomorpha in ZooKeys, 2015 (2015) 243-267.
Van De Kamp T.et al.: Three-dimensional reconstructions come to life – Interactive 3D PDF animations in functional morphology in PLoS ONE, 9 (2014) e102355.