Wulff N.C., Van De Kamp T., Dos Santos Rolo T., Baumbach T., Lehmann G.U.C.
in Scientific Reports, 7 (2017), 42345. DOI:10.1038/srep42345
© The Author(s) 2017. Male genital organs are among the fastest evolving morphological structures. However, large parts of the male’s genitalia are often hidden inside the female during mating. In several bushcricket species, males bear a pair of sclerotized genital appendices called titillators. By employing synchrotron-based in vivo X-ray cineradiography on mating couples, we were able to visualize titillator movement and spermatophore attachment inside the female. Titillators are inserted and retracted rhythmically. During insertion the titillator processes tap the soft and sensillae-covered dorsal side of the female’s flap-like genital fold, which covers the opening of the female’s genitalia, without tissue penetration. Titillators thus appear to be initially used for stimulation; later they may apply pressure that forces the female’s genital fold to stay open, thereby aiding mechanically in spermatophore transfer.
Cecilia A., Baecker A., Hamann E., Rack A., van de Kamp T., Gruhl F.J., Hofmann R., Moosmann J., Hahn S., Kashef J., Bauer S., Farago T., Helfen L., Baumbach T.
in Materials Science and Engineering C, 71 (2017) 465-472. DOI:10.1016/j.msec.2016.10.038
© 2016 Prostate cancer (PCa) currently is the second most diagnosed cancer in men and the second most cause of cancer death after lung cancer in Western societies. This sets the necessity of modelling prostatic disorders to optimize a therapy against them. The conventional approach to investigating prostatic diseases is based on two-dimensional (2D) cell culturing. This method, however, does not provide a three-dimensional (3D) environment, therefore impeding a satisfying simulation of the prostate gland in which the PCa cells proliferate. Cryogel scaffolds represent a valid alternative to 2D culturing systems for studying the normal and pathological behavior of the prostate cells thanks to their 3D pore architecture that reflects more closely the physiological environment in which PCa cells develop. In this work the 3D morphology of three potential scaffolds for PCa cell culturing was investigated by means of synchrotron X-ray computed micro tomography (SXCμT) fitting the according requirements of high spatial resolution, 3D imaging capability and low dose requirements very well. In combination with mechanical tests, the results allowed identifying an optimal cryogel architecture, meeting the needs for a well-suited scaffold to be used for 3D PCa cell culture applications. The selected cryogel was then used for culturing prostatic lymph node metastasis (LNCaP) cells and subsequently, the presence of multi-cellular tumor spheroids inside the matrix was demonstrated again by using SXCμT.
Zuber M., Laass M., Hamann E., Kretschmer S., Hauschke N., Van De Kamp T., Baumbach T., Koenig T.
in Scientific Reports, 7 (2017), 41413. DOI:10.1038/srep41413
© 2017 The Author(s). Non-destructive imaging techniques can be extremely useful tools for the investigation and the assessment of palaeontological objects, as mechanical preparation of rare and valuable fossils is precluded in most cases. However, palaeontologists are often faced with the problem of choosing a method among a wide range of available techniques. In this case study, we employ X-ray computed tomography (CT) and computed laminography (CL) to study the first fossil xiphosuran from the Muschelkalk (Middle Triassic) of the Netherlands. The fossil is embedded in micritic limestone, with the taxonomically important dorsal shield invisible, and only the outline of its ventral part traceable. We demonstrate the complementarity of CT and CL which offers an excellent option to visualize characteristic diagnostic features. We introduce augmented laminography to correlate complementary information of the two methods in Fourier space, allowing to combine their advantages and finally providing increased anatomical information about the fossil. This method of augmented laminography enabled us to identify the xiphosuran as a representative of the genus Limulitella.
Caselle M., Perez L.E.A., Balzer M., Kopmann A., Rota L., Weber M., Brosi M., Steinmann J., Brundermann E., Muller A.-S.
in Journal of Instrumentation, 12 (2017), C01040. DOI:10.1088/1748-0221/12/01/C01040
© 2017 IOP Publishing Ltd and Sissa Medialab srl. This paper presents a novel data acquisition system for continuous sampling of ultra-short pulses generated by terahertz (THz) detectors. Karlsruhe Pulse Taking Ultra-fast Readout Electronics (KAPTURE) is able to digitize pulse shapes with a sampling time down to 3 ps and pulse repetition rates up to 500 MHz. KAPTURE has been integrated as a permanent diagnostic device at ANKA and is used for investigating the emitted coherent synchrotron radiation in the THz range. A second version of KAPTURE has been developed to improve the performance and flexibility. The new version offers a better sampling accuracy for a pulse repetition rate up to 2 GHz. The higher data rate produced by the sampling system is processed in real-time by a heterogeneous FPGA and GPU architecture operating up to 6.5 GB/s continuously. Results in accelerator physics will be reported and the new design of KAPTURE be discussed.
Losel P., Heuveline V.
in Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 10129 LNCS (2017) 121-128. DOI:10.1007/978-3-319-52280-7_12
© Springer International Publishing AG 2017. Segmenting the blood pool and myocardium from a 3D cardiovascular magnetic resonance (CMR) image allows to create a patient-specific heart model for surgical planning in children with complex congenital heart disease (CHD). Implementation of semi-automatic or automatic segmentation algorithms is challenging because of a high anatomical variability of the heart defects, low contrast, and intensity variations in the images. Therefore, manual segmentation is the gold standard but it is labor-intensive. In this paper we report the set-up and results of a highly scalable semi-automatic diffusion algorithm for image segmentation. The method extrapolates the information from a small number of expert manually labeled reference slices to the remaining volume. While results of most semi-automatic algorithms strongly depend on well-chosen but usually unknown parameters this approach is parameter-free. Validation is performed on twenty 3D CMR images.
Steinmann J.L., Blomley E., Brosi M., Brundermann E., Caselle M., Hesler J.L., Hiller N., Kehrer B., Mathis Y.-L., Nasse M.J., Raasch J., Schedler M., Schonfeldt P., Schuh M., Schwarz M., Siegel M., Smale N., Weber M., Muller A.-S.
in Physical Review Letters, 117 (2016), 174802. DOI:10.1103/PhysRevLett.117.174802
© 2016 American Physical Society. Using arbitrary periodic pulse patterns we show the enhancement of specific frequencies in a frequency comb. The envelope of a regular frequency comb originates from equally spaced, identical pulses and mimics the single pulse spectrum. We investigated spectra originating from the periodic emission of pulse trains with gaps and individual pulse heights, which are commonly observed, for example, at high-repetition-rate free electron lasers, high power lasers, and synchrotrons. The ANKA synchrotron light source was filled with defined patterns of short electron bunches generating coherent synchrotron radiation in the terahertz range. We resolved the intensities of the frequency comb around 0.258 THz using the heterodyne mixing spectroscopy with a resolution of down to 1 Hz and provide a comprehensive theoretical description. Adjusting the electron’s revolution frequency, a gapless spectrum can be recorded, improving the resolution by up to 7 and 5 orders of magnitude compared to FTIR and recent heterodyne measurements, respectively. The results imply avenues to optimize and increase the signal-to-noise ratio of specific frequencies in the emitted synchrotron radiation spectrum to enable novel ultrahigh resolution spectroscopy and metrology applications from the terahertz to the x-ray region.
Bergmann T., Balzer M., Bormann D., Chilingaryan S.A., Eitel K., Kleifges M., Kopmann A., Kozlov V., Menshikov A., Siebenborn B., Tcherniakhovski D., Vogelgesang M., Weber M.
in 2015 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2015 (2016), 7581841. DOI:10.1109/NSSMIC.2015.7581841
© 2015 IEEE. The EDELWEISS experiment, located in the underground laboratory LSM (France), is one of the leading experiments using cryogenic germanium (Ge) detectors for a direct search for dark matter. For the EDELWEISS-III phase, a new scalable data acquisition (DAQ) system was designed and built, based on the ‘IPE4 DAQ system’, which has already been used for several experiments in astroparticle physics.
Harbaum T., Seboui M., Balzer M., Becker J., Weber M.
in Proceedings – 24th IEEE International Symposium on Field-Programmable Custom Computing Machines, FCCM 2016 (2016) 184-191, 7544775. DOI:10.1109/FCCM.2016.52
© 2016 IEEE. Modern high-energy physics experiments such as the Compact Muon Solenoid experiment at CERN produce an extraordinary amount of data every 25ns. To handle a data rate of more than 50Tbit/s a multi-level trigger system is required, which reduces the data rate. Due to the increased luminosity after the Phase-II-Upgrade of the LHC, the CMS tracking system has to be redesigned. The current trigger system is unable to handle the resulting amount of data after this upgrade. Because of the latency of a few microseconds the Level 1 Track Trigger has to be implemented in hardware. State-of-the-art pattern recognition filter the incoming data by template matching on ASICs with a content addressable memory architecture. An implementation on an FPGA, which replaces the content addressable memory of the ASIC, has not been possible so far. This paper presents a new approach to a content addressable memory architecture, which allows an implementation of an FPGA based design. By combining filtering and track finding on an FPGA design, there are many possibilities of adjusting the two algorithms to each other. There is more flexibility enabled by the FPGA architecture in contrast to the ASIC. The presented design minimizes the stored data by logic to optimally utilize the available resources of an FPGA. Furthermore, the developed design meets the strong timing constraints and possesses the required properties of the content addressable memory.
Rota L., Balzer M., Caselle M., Kudella S., Weber M., Mozzanica A., Hiller N., Nasse M.J., Niehues G., Schonfeldt P., Gerth C., Steffen B., Walther S., Makowski D., Mielczarek A.
in 2016 IEEE-NPSS Real Time Conference, RT 2016 (2016), 7543157. DOI:10.1109/RTC.2016.7543157
© 2016 IEEE. We developed a fast linear array detector to improve the acquisition rate and the resolution of Electro-Optical Spectral Decoding (EOSD) experimental setups currently installed at several light sources. The system consists of a detector board, an FPGA readout board and a high-Throughput data link. InGaAs or Si sensors are used to detect near-infrared (NIR) or visible light. The data acquisition, the operation of the detector board and its synchronization with synchrotron machines are handled by the FPGA. The readout architecture is based on a high-Throughput PCI-Express data link. In this paper we describe the system and we present preliminary measurements taken at the ANKA storage ring. A line-rate of 2.7 Mlps (lines per second) has been demonstrated.
Amstutz C. et al.
in 2016 IEEE-NPSS Real Time Conference, RT 2016 (2016), 7543110. DOI:10.1109/RTC.2016.7543110
© 2016 IEEE.The CMS collaboration is preparing a major upgrade of its detector, so it can operate during the high luminosity run of the LHC from 2026. The upgraded tracker electronics will reconstruct the trajectories of charged particles within a latency of a few microseconds, so that they can be used by the level-1 trigger. An emulation framework, CIDAF, has been developed to provide a reference for a proposed FPGA-based implementation of this track finder, which employs a Time-Multiplexed (TM) technique for data processing.