Schwermann A.H., dos Santos Rolo T., Caterino M.S., Bechly G., Schmied H., Baumbach T., van de Kamp T.
in eLife, 5 (2016), e12129. DOI:10.7554/eLife.12129
© Schwermann et al. External and internal morphological characters of extant and fossil organisms are crucial to establishing their systematic position, ecological role and evolutionary trends. The lack of internal characters and soft-tissue preservation in many arthropod fossils, however, impedes comprehensive phylogenetic analyses and species descriptions according to taxonomic standards for Recent organisms. We found well-preserved three-dimensional anatomy in mineralized arthropods from Paleogene fissure fillings and demonstrate the value of these fossils by utilizing digitally reconstructed anatomical structure of a hister beetle. The new anatomical data facilitate a refinement of the species diagnosis and allowed us to reject a previous hypothesis of close phylogenetic relationship to an extant congeneric species. Our findings suggest that mineralized fossils, even those of macroscopically poor preservation, constitute a rich but yet largely unexploited source of anatomical data for fossil arthropods.
Caselle M., Blank T., Colombo F., Dierlamm A., Husemann U., Kudella S., Weber M.
in Journal of Instrumentation, 11 (2016), C01050. DOI:10.1088/1748-0221/11/01/C01050
© 2016 IOP Publishing Ltd and Sissa Medialab srl.In the next generation of collider experiments detectors will be challenged by unprecedented particle fluxes. Thus large detector arrays of highly pixelated detectors with minimal dead area will be required at reasonable costs. Bump-bonding of pixel detectors has been shown to be a major cost-driver. KIT is one of five production centers of the CMS barrel pixel detector for the Phase I Upgrade. In this contribution the SnPb bump-bonding process and the production yield is reported. In parallel to the production of the new CMS pixel detector, several alternatives to the expensive photolithography electroplating/electroless metal deposition technologies are developing. Recent progress and challenges faced in the development of bump-bonding technology based on gold-stud bonding by thin (15 μm) gold wire is presented. This technique allows producing metal bumps with diameters down to 30 μm without using photolithography processes, which are typically required to provide suitable under bump metallization. The short setup time for the bumping process makes gold-stud bump-bonding highly attractive (and affordable) for the flip-chipping of single prototype ICs, which is the main limitation of the current photolithography processes.
Briceno R.D., Eberhard W.G., Chinea-Cano E., Wegrzynek D., Dos Santos Rolo T.
in Ethology Ecology and Evolution, 28 (2016) 53-76. DOI:10.1080/03949370.2014.1002114
© 2015 Dipartimento di Biologia, Università di Firenze, Italia. A long-standing question in morphological evolution is why male genitalia tend to diverge more rapidly than other structures. One possible explanation is that male genitalia are under sexual selection to function as internal courtship devices. Males of closely related species may provide divergent stimulation using different genital morphologies and behaviors. Testing this hypothesis has been difficult, however, because the presumed genital courtship behavior is often hidden from view inside the female, and because studies of how the males genitalia interact with those of the female are nearly always limited to a single species in a given group, thus restricting opportunities for comparison of closely related species. We present new morphological and behavioral data for portions of the male genitalia that are hidden in the female during copulation in five species in the tsetse fly genus Glossina using data from dissections of pairs frozen in copula, artificially stimulated males, and from copulating pairs viewed with a new X-ray technique that allows events inside the female to be recorded in real time. These data almost certainly give only an incomplete view of this complex, previously hidden world. But even so they clearly reveal that, as predicted by sexual selection theory, the male genitalia of Glossina flies perform dramatic, stereotyped, rhythmic movements deep within the females reproductive tract and in inward folds of her external surface, and that many of these movements probably differ among closely related species. Most of the movements are not explicable as means by which the male anchors himself more securely to the female; all are likely to result in stimulation of the female. A female Glossina can be stimulated tactilely at a given moment during copulation at up to 8-10 or more different sites on her body.
Mangold S., Van De Kamp T., Steininger R.
in Journal of Physics: Conference Series, 712 (2016), 012141. DOI:10.1088/1742-6596/712/1/012141
The usefulness of full field transmission spectroscopy is shown using the example of mandible of the stick insect Peruphasma schultei. An advanced data evaluation tool chain with an energy drift correction and highly reproducible automatic background correction is presented. The results show significant difference between the top and the bottom of the mandible of an adult stick insect.
van de Kamp T., Riedel A., Greven H.
in Arthropod Structure and Development, 45 (2016) 14-22. DOI:10.1016/j.asd.2015.10.002
© 2015 Elsevier Ltd.The elytral cuticle of 40 beetle species, comprising 14 weevils (Curculionoidea) and 26 representatives of other taxa, is examined. All weevils and 18 other species have an endocuticle with prominent macrofibers, which corresponds to a modified pseudo-orthogonal cuticle. Angles between successive layers of macrofibers range between 30° and 90°, but are constantly less than 60° in weevils. In all Curculionoidea, as well as in one buprestid and one erotylid species exo- and endocuticle are densely interlocked. In the weevil Sitophilus granarius, transmission electron microscopy revealed vertical microfibrils extending from the exocuticle between the macrofibers of the underlaying endocuticle. Vertical microfibrils connecting successive macrofiber layers of the endocuticle were observed in S. granarius and Trigonopterus nasutus. Distinct cuticular characters are traced on a beetle phylogeny: the angles between unidirectional endocuticle layers; the presence and the shape of endocuticular macrofibers; and the interlocking of exo- and endocuticle. While character traits seem to be more or less randomly distributed among Coleoptera, the Curculionoidea have a uniform groundplan: The “weevil-specific” combination of characters includes 1) interlocking of exo- and endocuticle, 2) an endocuticle with distinct ovoid macrofibers embedded in a matrix and 3) comparatively small angles between successive endocuticular layers. Thus, phylogenetic constraints appear equally important to functional factors in the construction of the weevil elytron.
Bruckner A., Wehner K., Neis M., Heethoff M.
in Acarologia, 56 (2016).
© Brückner A. et al. Oribatid mites represent a diverse group of soil micro-arthropods. They have evolved a broad range of defensive chemical and morphological traits (e.g. sclerotization, ptychoidy, biomineralization). Chemical defense, rather than sclerotization, can provide protection against large predators (staphylinid beetles) and many oribatid mite species are also well protected against gamasid soil mites using morphological traits (“enemy-free-space hypothesis”). However, since predatory mites and staphylinid beetles have different types of attacking and feeding, the adaptive values of chemical and morphological traits might differ accordingly. We used the oribatid model species Archegozetes longisetosus Aoki and the common gamasid mite Stratiolaelaps miles Berlese in a predator-prey experiment. We tested for effects of chemical defense (treatments with and without oil gland secretions) and sclerotization (treatments with unsclerotized tritonymphs and sclerotized adults) in an orthogonal design. In contrast to attacks by large predators, chemical defense was mostly ineffective against gamasid mites. Sclerotization, however, had a positive effect. Hence, in a natural environment with diverse types of predators, the “enemy-free space” seems only realizable by combinations of chemical and morphological protective traits.
Steinmann J.L., Blomley E., Brosi M., Brundermann E., Caselle M., Hiller N., Kehrer B., Muller A.-S., Schedler M., Schonfeldt P., Schuh M., Schwarz M., Siegel M.
in IPAC 2016 – Proceedings of the 7th International Particle Accelerator Conference (2016) 2855-2857.
Copyright © 2016 CC-BY-3.0 and by the respective authors. We present the effects of the filling pattern structure in multi-bunch mode on the beam spectrum. This effects can be seen by all detectors whose resolution is better than the RF frequency, ranging from stripline and Schottky measurements to high resolution synchrotron radiation measurements. Our heterodyne measurements of the emitted coherent synchrotron radiation at 270 GHz reveal discrete frequency harmonics around the 100 000th revolution harmonic of ANKA, the synchrotron radiation facility in Karlsruhe, Germany. Significant effects of bunch spacing, gaps between bunch trains and variations in individual bunch currents on the emitted CSR spectrum are described by theory and supported by observations.
Losel P., Heuveline V.
in Progress in Biomedical Optics and Imaging – Proceedings of SPIE, 9784 (2016), 97842L. DOI:10.1117/12.2216202
© 2016 SPIE. Inspired by the diffusion of a particle, we present a novel approach for performing a semiautomatic segmentation of tomographic images in 3D, 4D or higher dimensions to meet the requirements of high-throughput measurements in a synchrotron X-ray microtomograph. Given a small number of 2D-slices with at least two manually labeled segments, one can either analytically determine the probability that an intelligently weighted random walk starting at one labeled pixel will be at a certain time at a specific position in the dataset or determine the probability approximately by performing several random walks. While the weights of a random walk take into account local information at the starting point, the random walk itself can be in any dimension. Starting a great number of random walks in each labeled pixel, a voxel in the dataset will be hit by several random walks over time. Hence, the image can be segmented by assigning each voxel to the label where the random walks most likely started from. Due to the high scalability of random walks, this approach is suitable for high throughput measurements. Additionally, we describe an interactively adjusted active contours slice by slice method considering local information, where we start with one manually labeled slice and move forward in any direction. This approach is superior with respect to accuracy towards the diffusion algorithm but inferior in the amount of tedious manual processing steps. The methods were applied on 3D and 4D datasets and evaluated by means of manually labeled images obtained in a realistic scenario with biologists.
Vogelgesang M., Rota L., Perez L.E.A., Caselle M., Chilingaryan S., Kopmann A.
in Proceedings of SPIE – The International Society for Optical Engineering, 9967 (2016), 996715. DOI:10.1117/12.2237611
© Copyright 2016 SPIE. With ever-increasing data rates due to stronger light sources and better detectors, X-ray imaging experiments conducted at synchrotron beamlines face bandwidth and processing limitations that inhibit efficient workflows and prevent real-time operations. We propose an experiment platform comprised of programmable hardware and optimized software to lift these limitations and make beamline setups future-proof. The hardware consists of an FPGA-based data acquisition system with custom logic for data pre-processing and a PCIe data connection for transmission of currently up to 6.6 GB/s. Moreover, the accompanying firmware supports pushing data directly into GPU memory using AMD’s DirectGMA technology without crossing system memory first. The GPUs are used to pre-process projection data and reconstruct final volumetric data with OpenCL faster than possible with CPUs alone. Besides, more efficient use of resources this enables a real-time preview of a reconstruction for early quality assessment of both experiment setup and the investigated sample. The entire system is designed in a modular way and allows swapping all components, e.g. replacing our custom FPGA camera with a commercial system but keep reconstructing data with GPUs. Moreover, every component is accessible using a low-level C library or using a high-level Python interface in order to integrate these components in any legacy environment.
Ametova E., Ferrucci M., Chilingaryan S., McCarthy M., Dewulf W.
in Proceedings – ASPE 2016 Annual Meeting (2016) 287-292.